RIPK1 and colorectal adenocarcinoma: In two human colorectal adenocarcinoma lines, HT-29 and COLO 205, that are sensitive to necroptosis induced by treatment with TNFα, zVAD-fmk, and cIAP inhibitor BV6, multiple sgRNAs targeting PTBP1 enhance inclusion of the RIPK1 alternative exon (Fig. 5a–d).