Herein, we hypothesized that cinacalcet HCl directly modulates the upstream AMPK kinases such as [Ca2+]i, CaMKKα/β and/or LKB1, and the downstream signaling PGC-1α-eNOS-NO system to prevent and ameliorate DN in type 2 diabetes. The gene discussed is PRKAA1; the disease is liver dysplastic nodule.