To test the effects of KRas on endogenous levels of PEAK1 in CRC cells, we used qRT-PCR and western blot assays to measure the mRNA and protein levels of PEAK1 in HCT 116 and CaCO2 cells infected with a pLenti- KRas or siRNA-KRas. The gene discussed is PEAK1; the disease is colorectal carcinoma.