EPHA2 and hepatocellular carcinoma: We then constructed an EphA2-luciferase reporter by inserting the 2000-bp 5′ promoter region of EphA2 containing TR4REs into the PGL3 luciferase plasmid (Fig. 4e) and tested whether the expression of this promoter-mediated luciferase activity could be changed after altering TR4 expression in HCC LM3-WPITR4 cells and Huh7-shTR4 cells.