TKI‐sensitive cells (PC‐9P), together with TKI‐resistant (PC‐9GR and H1975) NSCLC and NL20 cells, immortalized non‐transformed lung cells carrying wild‐type EGFR, were treated with Gefitinib to determine whether response to TKIs was linked to EGFR palmitoylation. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.