Twelve canonical pathways were enriched in all four contrasts from the second year (Calcium Signaling, The Visual Cycle, Tight Junction Signaling, Cellular Effects of Sildenafil, Epithelial Adherens Junction Signaling, Sertoli Cell Junction Signaling, Maturity Onset Diabetes of Young Signaling, ILK Signaling, Leukocyte Extravasation Signaling, FXR/RXR Activation, Clathrin-mediated Endocytosis Signaling, and Germ Cell-Sertoli Cell Junction Signaling) suggesting enrichment for sex-biased genes in pathways involved with cell signaling, and sexual development. Here, ILK is linked to diabetes mellitus.