Likewise, these markers were also confirmed by immunohistochemical staining in serial sections of ICC tissues, and in the positive TRIM44 staining of ICC tissues, downregulaton of E‐cadherin and up‐regulation of vimentin, β‐catenin and snail were observed in invasive tumor fringe and vice versa (Fig. 4D). The gene discussed is SNAI1; the disease is intrahepatic cholangiocarcinoma.