To better understand the prognostic value of TRIM44 in ICC patients, we further analyzed by dividing all the patients into several subgroups, and observed that predictive value of TRIM44 were continued in tumor size (<5 cm, P < 0.001), single tumor (P < 0.001), no embolus (P = 0.001), no encapsulation (P < 0.001), no metastasis (P = 0.0069), and high differentiation subgroups (P = 0.004; Fig. 6C). This evidence concerns the gene TRIM44 and intrahepatic cholangiocarcinoma.