Using RBBP8 as a methylation biomarker, BLCA could be detected with a 25% sensitivity (at maximal specificity of 100%) in urines from BLCA patients, i.e., enabling to distinguish early cancerous (CIS), high-grade urothelial, and even papillary subtype tumors, which are difficult to detect by urine cytology, from non-cancerous and benign ones. This evidence concerns the gene RBBP8 and in situ carcinoma.