Figure 8(e) demonstrates that the decreased c-kit in constipation rats significantly increased by 31.2% after treating the rats with 500 μg/ml AEHC (P < 0.05). In addition, treatment with 100 and 200 μg/ml AEHC elevated c-kit protein expression levels. SCF protein expression significantly increased by 20.1, 24.7, and 8.4% at treatment with 100, 200, and 500 μg/ml AEHC, respectively (Figure 9). This evidence concerns the gene KIT and Constipation.