To confirm our findings, we genotyped the associated SNPs on an independent dataset composed of 52 cases with tooth agenesis and 427 gender- and ethnicity-matched unrelated controls, and found significant association between CASC8 rs10505477 (P = 0.006 for genotype and P = 0.008 for allele) and TA, particularly under a dominant model (P = 0.001). This evidence concerns the gene CASC8 and tooth agenesis.