CHUK and cancer: To define the NF-κB-activating kinase responsible for aberrant NF-κΒ signaling of KRASMUT cancer cells, we stably expressed shRNAs specifically targeting IKKα, IKKβ, IKKε, and TBK1 transcripts (Chuk, Ikbkb, Ikbke, and Tbk1, respectively) in our pNGL-expressing cell lines and validated them (Fig. 5a).