Considering that MHCC97L and HCCLM3 (metastatic potential: MHCC97L < HCCLM3) were established from the same parent HCC cell line and showed relatively high and low CALML3 expression respectively, we established a doxycycline-inducible CALML3 overexpression system in HCCLM3 and a CRISPR/Cas9-mediated knockout in MHCC97L cells. Here, CALML3 is linked to hepatocellular carcinoma.