The result of univariate analysis showed that intratumoural CALML3 expression, tumour encapsulation, vascular invasion, tumour number, tumour size, Edmondson grade, BCLC stage, ALT (alanine aminotransferase), TB (total bilirubin) and AFP (alpha fetoprotein) were significantly associated with OS in univariate analyses. The gene discussed is AFP; the disease is neoplasm.