To explore relationship between dysfunctional CALML3 and metastasis potential in liver cancer cells, we studied the expression profile of CALML3 in human hepatocyte cell line THLE-3 and seven hepatoma cell lines with different metastatic potentials, including cells with no or low metastatic potential (HepG2, Bel-7402, SMMC-7721 and PLC/PRF/5) and serial cell lines with medium or high metastatic potential (MHCC97L, MHCC97H and HCCLM3). This evidence concerns the gene CALML3 and hepatocellular carcinoma.