For this, we used a polymerase chain reaction (PCR) array including 84 typical cancer-associated genes (e.g., ones involved in cell adhesion, extracellular matrix (ECM) components, cell cycle, cell proliferation and apoptosis) and five housekeeping genes (as controls), to validate CALML3-regulated DEGs in wild-type HCCLM3 cells (HCCLM3/NC) and CALML3 overexpression HCCLM3 cells (HCCLM3/CALML3) (Supplementary Data 5). This evidence concerns the gene CALML3 and cancer.