Additionally, compared with WT mice, FGF21 deficiency further decreased the phosphorylation of cardiac AKT (Fig. S20A) and GSK-3β (Fig. S20B) induced by T2DM, associated with the upregulation of TRB3 expression (Fig. S20C) and PTEN activity (Fig. S20D) which are the negative regulators of AKT. This evidence concerns the gene AKT1 and type 2 diabetes mellitus.