These results suggest that cytotoxicity of Nigella sativa seed oil and methanolic extract, etoposide, and OM-90 in monotherapy and in particular combination of Nigella sativa seed oil used together with OM-90 or etoposide and Nigella sativa seed extract with OM-90 or etoposide against AGS stomach cancer cells is mainly due to inducing apoptotic cell death (as well as necrotic cell death), which is in accordance with the results observed in the above Annexin V/PI assay. The gene discussed is ANXA5; the disease is gastric cancer.