Presence of plasma circulating tumor DNA (ctDNA) has higher positive predictive value than six biomarkers [CA125, CA19-9, CYFRA21-1, CEA, NSE, squamous cell carcinoma antigen (LSCC)]. Concordance rate between tDNA and ctDNA mutations was 78.1%. Decrease in AF of plasma ctDNA mutations observed 2 days postoperatively. This evidence concerns the gene ENO2 and neoplasm.