Serum cfDNA was analyzed for promoter methylation of six genes (SFN, P16, hMLH1, HOXD13, PCDHGB7, and RASSF1a) by the qPCR-based MethyLight test in 749 patients with BC, benign breast lesions, and healthy subjects for early disease detection (68). The gene discussed is MLH1; the disease is breast cancer.