Compared with lane 2 (TBK1 + FLAG), immunoblotting analysis of anti-FLAG immunoprecipitate with an anti-IRF3 antibody showed that TBK1_tv2 but not TBK1_tv1 decreased the interaction between TBK1 and endogenous IRF3 protein during SVCV infection (lanes 5–6 in Figure 8A). Here, TBK1 is linked to infection.