To determine the inhibitory role of TBK1_tv1 and TBK1_tv2 in the complex of TBK1 and endogenous IRF3 protein in response to viral infection, EPC cells were cotransfected with TBK1-FLAG together with TBK1_tv1-GFP or TBK1_tv2-GFP, and then infected with SVCV. Here, TBK1 is linked to viral infectious disease.