Now, based on the results from TLGS brain tissue, we have confirmed in the human neuroblastoma cell line SHSY5Y that the expressions of the miR-200 and miR-182 clusters are regulated by the Akt1/Akt2 ratio, and furthermore that overexpression or depletion of these miRNAs caused differential expression of EMT-related proteins. The gene discussed is AKT1; the disease is neuroblastoma.