Furthermore, by contrast, knockdown of DEPP expression with two different targeting siRNA significantly prevented senescence induction (SA-β-Gal+ cells changed from 28.3 to 13.4 and 11.2%, respectively), decreased the S-phase cell cycle arrest and increased the numbers of cells in G0/G1 phase, as well as increased the number of colonies formed by HCT116 colon cancer cells (Fig. 5b–g). The gene discussed is DEPP1; the disease is malignant colon neoplasm.