The evaluation of the chondrogenic differentiation was assessed by RT-qPCR for genes that encode proteins characteristic of native hyaline cartilage: Col2a1, Acan, and Snorc. Col1a1 and HtrA1 encode respectively a non-specific protein and a protease induced in osteoarthritis (OA) cartilage. The gene discussed is ACAN; the disease is osteoarthritis.