Finally, we anticipate that the nucleic acid‐free, soluble recombinant full‐length TDP‐43 fusion protein and the TDP‐43 phase separation assays described here will be of use in the future for evaluating the mechanistic effect of ALS variants, RNA interaction, and intermolecular interactions that give rise to TDP‐43 function. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.