MYH9 and acute kidney injury: Immunoblot assays demonstrated that the expression levels of the MYH9 gene in A+/AKI EF1α-GFP-pA, A+/AKI pA-GFP-EF1α ES cells are completely comparable to that in A+/A+ ES cells, suggesting little or no effect of the inserted exogenous genes at the intron 2 site on the transcription of the MYH9 gene (Fig 5).