To confirm the loss of CTCF binding during in vivo infection due to deletion of the CTRL2 site and to validate the mutant virus, we infected mice via corneal scarification with 2 × 105 PFU/eye of ΔCTRL2 or CTRL2R virus and performed chromatin immunoprecipitation (ChIP) for CTCF. The gene discussed is CTCF; the disease is infection.