One possible explanation is that the p53 gene mutations and loss of p53 gene heterozygosity are common in HCC, especially HCC with a heavy tumor burden, which is reflected by the elevated APF values and enlarged tumors.46,47 H101 may be more effective for tumors in which p53 gene mutations or deletions are more frequent.48 What is more, blood flow is abundant in HCC with a heavy tumor burden.49 Higher H101 concentrations in vascular-rich areas where tumor cells grow faster may inhibit tumor cell growth in a timely and effective manner. This evidence concerns the gene TP53 and hepatocellular carcinoma.