Followed by in vitro luciferase reporter assay, miR-193b-3p was detected as a direct negative regulator of MYB. Remarkably, downregulation of miR-193b-3p by antagomiR in the NOTCH1-induced mouse model significantly accelerated leukemia onset in vivo, suggesting the inhibition of tumor-suppressor miR-193b-3p can cooperate with NOTCH1 in T-ALL pathogenesis. This evidence concerns the gene MYB and neoplasm.