Next, some of the genes identified through the expression array (HAS2, Sox4, MUC1) along with selected genes (PLAU, SPARC, FABP7, HAS3) implicated in cancer progression and metastasis were subjected to quantitative polymerase chain reaction (qPCR) analysis using the total RNA from control and 200 μM SAM-treated MDA-MB-231 cells. This evidence concerns the gene SPARC and cancer.