The A549 cells (1 × 106 cells per 0.1 mL) were infected with/without the miR-30a lentiviral expression plasmid (miR-30a), SIRT1 plasmid(SIRT1), or miR-30a lentiviral expression plasmid plus SIRT1 overexpression plasmid(miR-30a+SIRT1); then, the cells were implanted subcutaneously into 6-week-old nude mice and tumor growth was measured after 25 days. Here, SIRT1 is linked to neoplasm.