Interestingly, our data demonstrate robust Shh mRNA expression levels in IMR90 fibroblasts (compared to negative OCUT1 and CAL62 cancer cells) (Figure 5A) and high-level secretion of Shh ligand (measured through an ELISA assay) in IMR90 and thyroid MSCs as compared to non-detectable values in OCUT1 and CAL62 cells (Figure 5B). Here, SHH is linked to cancer.