Here we evaluated by immunohistochemistry Gli1 expression levels in a panel of 66 human tissues including: five normal thyroids, 11 benign adenomas, 10 papillary thyroid cancers (PTCs), 10 medullary thyroid cancers (MTCs), 14 follicular thyroid cancers (FTCs), and 16 anaplastic thyroid cancers (ATCs). This evidence concerns the gene GLI1 and adenoma.