Considering that the reports on dual-targeted systems with Tf and CPP, in ovarian cancer, are limited, we hypothesized that surface-modification of DOX-loaded liposomes with R8 and Tf (Dual DOX-L), will improve selectively of the liposomes toward the over-expressed TfRs and help in better cyotosolic DOX delivery leading to enhanced anti-cancer effects both in vitro and in vivo. The gene discussed is TF; the disease is ovarian cancer.