However compound containing a hydroxyl substitution at the 8th position (2i) showed significant selectivity towards ER(+) MCF-7 breast cancer cell lines compared to ER(−) MDA-MB-231 cell lines indicating that this particular compound may be acting via ER dependent mechanism while compounds (2b) and (3g) may be acting via ER independent mechanisms. The gene discussed is ESR1; the disease is breast cancer.