Correlating additional epigenetic enzyme expression (IDH1/2, DNMT1, DNMT3A/B) and mean tumor 5hmC β-values demonstrated a significant negative correlation between DNMT3B expression and 5hmC when considering loci of high 5hmC abundance (top 1% probes, rs = − 0.603, p value = 0.0049) (suppl. Here, DNMT3B is linked to neoplasm.