BRAF and melanoma: Of note, alterations in regions harbouring typical melanoma-driving onco- and tumour suppressor genes such as BRAF or CDKN2A (9p21-p24) emerged at a DCCD of 77–106, exactly when we noted morphological colony formation (Fig. 7b and Supplementary Tables 2 and 3). NRAS mutation was associated with insignificant classification FDR values and the best classifier for NRAS separated out only 4 out of 87 samples.