Consistent with echocardiography, the protective effect of 2 ME against AAC induced cardiac hypertrophy was associated with a substantial increase in the expression of cardioprotective proteins, protein FAM65B and vinculin, and a significant decrease in the creatine kinase B-type protein, a biomarker of heart disease, by 3.9, 1.33 and 0.44 fold of change, respectively, in comparison to AAC group (Table 3). Here, VCL is linked to cardiac hypertrophy.