IFNB1 and infection: While wild-type L929 cells markedly induced ISRE-IFNβ promoter-luciferase expression upon HSV-1ΔICP34.5 infection, TRIM56(+) cGAS(−), TRIM56(−) cGAS(+), or TRIM56(−) cGAS(−) L929 cells showed defective ISRE-IFNβ promoter-luciferase expression under the same conditions (Fig. 2f).