We thus proposed that levels of CEA, CA19-9, CA72-4, and CA125 can be used as the biomarkers to not only diagnose CRC, but also predict CRC lymph node metastasis, vascular invasion, neural infiltration, tumor differentiation and staging, therefore to be used to guide the treatment and prognosis of CRC. This evidence concerns the gene MUC16 and colorectal carcinoma.