Finally, in comparison to the overweight-without-NAFLD group, the lean with NAFLD group had significantly higher hepatic triglyceride content, HOMA-IR, and serum fasting insulin, AST and ALT values and significantly lower serum adiponectin values (using one-way ANOVA for continuous variables followed by the Tukey multiple-comparison test). This evidence concerns the gene INS and metabolic dysfunction-associated steatotic liver disease.