An appreciable number of false-negative results are expected with these methods, given the large proportion of individuals with NAFLD who have “normal” serum aminotransferase levels, alanine aminotransferase (ALT) <40 IU [1,21,22] and the poor sensitivity of abdominal ultrasound to detect mild steatosis (liver fat content<30%) [12,13]. The gene discussed is GPT; the disease is steatosis.