To validate the results of PET/CT/MR imaging with 12[18F]DDAHA and to assess the cell specificity and heterogeneity of SIRT2 expression-activity in 9L gliomas and in different brain structures, we used immunofluorescent microscopy (IFM) in 9L glioma-bearing rat brain tissue sections. This evidence concerns the gene SIRT2 and central nervous system cancer.