TGFB1 and glioblastoma: To further investigate whether PF causes anti-EMT effects by inhibition of TGFβ in glioblastoma cells, we generated stable transfected U87, U251 and T98G cell lines overexpressing TGFβ by transfection of a lentiviral vector carrying eGFP tagged TGFβ cDNA and selected by puromycin.