After 7 days of co-culture of MPN-BMSCs with CB CD34+ cells, ASXL1 overexpression in MPN-BMSCs restored their effect on CB CD34+ cell differentiation as evidenced by the reduced percentage of myeloid cells (CD45+/CD33+) and increased percentage of erythroid cells (CD71+/CD235a+) compared with EV-transduced MPN-BMSCs (Supplementary Fig. S1j, k). This evidence concerns the gene CD33 and myeloproliferative neoplasm.