TUFT1 and lung adenocarcinoma: We observed high expression of TUFT1 in TTF-1-negative A549 lung adenocarcinoma cells compared to TTF-1-positive NCI-H441 cells (Supplementary Figure S1C); furthermore, TUFT1 expression was strongly induced by TGF-β and the protein was located mainly in the cytoplasm (Fig. 1b and Supplementary Figure S1D).