Regarding the strong effects of rigosertib on HCC cell lines in vitro (Figures 1–3), qRT-PCR analysis of HRAS and PLK1 expression levels was also performed in HCC cell lines (HepG2, Hep3B, PLC, Huh-7) as compared to primary human hepatocytes (PHH), and revealed marked overexpression of both HRAS (Figure 4E) and PLK1 (Figure 4F) in HCC cells. This evidence concerns the gene PLK1 and hepatocellular carcinoma.