SMAD4 and colorectal cancer: These included genes recurrently mutated in colorectal cancer such as APC, TP53, KRAS, EGFR, SMAD4, FBXW7, NRAS, VEGFA and RNF43. The genes affected most frequently by discordant copy number status between primary and paired metastatic tissues were CSMD3, TRPS1, TGFBR2, CTNNB1, FHIT and MACROD2 (Table 3).