In the present study, we have found that beyond the SP1 binding cluster, TFCP1/ZEB1 binding site which covers three CpG sites following the SP1 cluster seems to have a higher methylation rate in normal esophageal epithelial tissues and some MAGE-A11 low-expressed ESCC cells than the SP1 cluster. Here, SP1 is linked to esophageal squamous cell carcinoma.