In Eca109 cells, the specific binding of TFCP2 to MAGE-A11 promoter was only slightly increased as compared with IgG control (1.72 of fold enrichment), whereas DAC treatment could increase the specific binding of TFCP2 to MAGE-A11 promoter in Eca109 cells (9.63 of fold enrichment), suggesting that TFCP2 could directly bind to MAGE-A11 promoter in a methylation-dependent manner in ESCC cells. This evidence concerns the gene TFCP2 and esophageal squamous cell carcinoma.