To characterize the CML cell line K562, we sorted the cells into CD34+/CD38− and CD34+/CD38+ populations using the FACS-based regions, after staining the cells with CD38-PE and CD34-FITC, with Isotype-PE and Isotype-FITC as controls. This evidence concerns the gene CD34 and chronic myelogenous leukemia, BCR-ABL1 positive.