To study the effect of the TLR ligands on the differentiation of the cell lines, we performed the immunophenotyping of cells incubated for 72 h in the presence or absence of each ligand, by staining cells with the EuroFlowTM antibody panel for AML (HLADR, CD45, CD16, CD35, CD13, CD64, CD34, CD117, CD11b, CD33, CD10 and CD14). Here, ANPEP is linked to acute myeloid leukemia.