When only the definite TB patients were compared to ORD, the AUCs for individual analytes ranged from 0.52 (95% CI, 0.45–0.60) for unstimulated IL-1ra, to 0.75(95% CI, 0.70–0.80) for unstimulated IFN-γ (see Supplementary Table S2), thereby confirming the limited utility of individual analytes, and also suggesting that Mtb antigen stimulation might not be necessary for optimal diagnosis of TB disease. The gene discussed is IFNG; the disease is tuberculosis.