After we confirmed significant reduction in Wgn transcript levels in larval progeny of Wgn-RNAi flies crossed with Actin-Gal4 compared to larval progeny of w1118 flies crossed with this driver line (Figure A2), we found significantly higher survival rates for larvae expressing the Wgn-RNAi construct compared to their controls following infection with axenic nematodes (p = 0.005; Figure 1C). This evidence concerns the gene LGALS4 and infection.