In hepatocellular carcinoma (HCC), JNK has been linked to both the pathogenesis and poor prognosis of the disease.12 In this study, lentiviral approaches were used to stably knockdown JNK1, JNK2, and JNK1/2 in the HuH‐7 HCC cell line to investigate the effects of JNK knockdown on cell proliferation. This evidence concerns the gene MAPK9 and hepatocellular carcinoma.