FFA elevation, obtained by Intralipid/heparin infusion, negated the insulin effect on AT SIRT1. This negation of insulin effect may seem paradoxical, when we take into account that a high-fat diet decreases AT SIRT1 [37], however, it may reflect insulin-desensitizing action of FFA and may indicate that AT SIRT1 response contributes to FFA-induced insulin resistance. The gene discussed is SIRT1; the disease is Insulin resistance.