GPC3 and hepatocellular carcinoma: Hepa1-6 TEXs express two well-characterized HCC antigens-alpha-fetoprotein (AFP) and glypican 3 (GPC3), and can be taken up by DCs, thereby promoting DC maturation through enhancing the expression levels of CD11c, MHC, co-stimulatory factors (CD80, CD86), and intercellular adhesion molecule (ICAM) [12].