None of the reference parameters such as location, distance from skin, number, shape, margin, background echotexture, posterior features, BI-RADS category, age, ER, PR, HER-2 or Ki-67 were statistically significant in predicting ALNM in invasive ductal breast carcinoma with sizes > 10 mm (all P > 0.05) (Supplementary Table 1). The gene discussed is MKI67; the disease is invasive ductal breast carcinoma.